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with many different substrate analogs. The response system is of curiosity because NagA from bacterial sources is recognized with both a number of divalent metallic ions within the Energetic Net-web site.
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The steel content material in addition to the kinetic parameters in your mutants of NagA created for this investigation are available in Desk four.
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Mutation of Y223 into a phenylalanine residue resulted inside a mutant with just about precisely the same catalytic motion because the wild kind enzyme and so this residue just is just not significant for that binding of substrate in NagA.
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